Genomic Projects, Resources
- 测序技术和测序对象的发展趋势
- Gene annotation
- Other projects
- Metagenomics: Environmental, Organismal
- Ancient DNA
- Database and Server
- EMBL
- GenBank
- DDBJ
金标准：Refseq，NCBI Reference Sequences
Protein Sequence Database：
- swiss-prot
- TrEMBL
- Uniprot
Organism Specific Database 针对特定物种的database
NGS
Next Generation Sequencing 第二代测序技术
第一代测序技术：Sanger sequencing
第二代测序技术：NGS
Pair-end sequencing
第三代测序技术：PacBio, Oxford
Types of NGS:
- DNA-seq
- Library preparation
- Sequencing
- RNA-seq
- Epigenetics
- Interaction
Targeted Sequencing
- Hybrid capture
- Amplicon sequencing
Example Study
Exosome sequencing of cancer samples
DNA-seq steps:
- Quality control
- Reads mapping
- Mutations Identification
- Visualization
Genomic Features
C-value
GC content
Non-coding RNAs/Genes
Pseudogenes
Genome Structure